Journal of Dental Research, Vol 64, 1199-1203, Copyright © 1985 by International & American Associations for Dental Research Online Journals
Comparison of cyclic AMP-dependent protein kinases from salivary glands of four species
F. Dowd, P. Cheung, J. Warren, T. Faerber and D. Traub
Cyclic AMP-dependent protein kinase activity, subcellular distribution, and
isozyme profile were compared in rabbit, rat, guinea pig, and mouse in both
parotid and submandibular glands. Glands were homogenized under hypotonic
conditions and the following fractions isolated: 600 g pellet, 27,000 g
pellet, and 27,000 g supernatant. The specific activity of the enzyme was
similar in the eight glands and was highest in the 27,000 g supernatant.
The average activity in the 27,000 g supernatant was approximately 75% of
the total gland activity, although there was considerable variability
between tissues and species. After being washed with isotonic buffer, this
percentage was increased to an average of 84%. When isozyme patterns of the
kinase were examined, the rabbit parotid was unique in that it contained a
high percentage of isozyme I as isolated on DEAE cellulose columns.
