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Journal of Dental Research, Vol 64, 1195-1198, Copyright © 1985 by International & American Associations for Dental Research Online Journals


ARTICLES

Predominant obligate anaerobes in human carious dentin

E. Hoshino

With the adoption of an anaerobic glove box system, we isolated and identified the predominant micro-organisms in lesions of carious dentin from four permanent third molars. The overwhelming majority of the micro-organisms isolated from both shallow and deep layers of carious dentin were obligate anaerobes, suggesting that conditions in the dentin were strictly anaerobic. The predominant obligate anaerobes were Gram-positive rods which were identified as members of the following genera: Propionibacterium, Eubacterium, Arachnia, Lactobacillus, Bifidobacterium, and Actinomyces. Clostridia, Gram-negative rods (Bacteroides and Fusobacterium) and Gram-positive cocci (Peptococcus, Peptostreptococcus, and Streptococcus) were also isolated, although they constituted a minor part of the flora.


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