Journal of Dental Research, Vol 63, 1116-1120, Copyright © 1984 by International & American Associations for Dental Research Online Journals
Incisional biopsy and seeding in hamster cheek pouch carcinoma
I. M. Safour, N. K. Wood, K. Tsiklakis, D. B. Doemling and G. Joseph
Surgical blades were contaminated by doing incisional biopsies on
DMBA-induced cheek pouch carcinomas in hamsters. Contaminated blades were
then used to: (1) make cytologic smears; (2) make incisions in the mucosal
surface of the lower lip; (3) make incisions in the ventral surface of the
tongue; (4) make incisions in the untreated left cheek pouch; and (5) make
incisions in the pre-shaved back. One group of animals was killed three hr
post-surgery. The second group was killed seven wk post-surgery. Tissues
were excised and processed for microscopic study. Malignant cells were
found in many of the incision sites of animals from the first group. In the
animals of the second group, several tumors growing from the transplanted
tumor cells were observed in the incision sites of the lower lips and the
tongue. None was observed in the incision sites of the left cheek pouch or
the back. Cervical lymph node metastases were found in approximately 50% of
the animals of the second group.
