Journal of Dental Research, Vol 63, 94-97, Copyright © 1984 by International & American Associations for Dental Research Online Journals
Rat intestinal vitamin D-dependent calcium-binding protein: immunocytochemical localization in incisor ameloblasts
A. N. Taylor, W. A. Gleason Jr and G. L. Lankford
Antiserum raised against the 10,000-Dalton vitamin D-dependent
calcium-binding protein (CaBP10) from rat intestine was used to localize
CaBP10 immunocytochemically in histological sections of rat mandible using
an indirect immunoperoxidase method. Ameloblasts in the zone of maturation,
along the continuously erupting incisor, contained CaBP10 throughout their
cytoplasm. It was present in both smooth-ended ameloblasts and ruffle-ended
ameloblasts. CaBP10 was not found in earlier developmental stages of
ameloblasts or in other cells involved in tooth formation, i.e.,
odontoblasts, pulpal cells, cells of the stellate reticulum, papillary
layer, and outer dental epithelium. The presence of CaBP10 in ameloblasts
suggests that the vitamin D-endocrine system may have a direct effect on
tooth formation in addition to the indirect effect of maintaining the
required levels of serum calcium and phosphorus required for
mineralization.
