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Journal of Dental Research, Vol 63, 111-113, Copyright © 1984 by International & American Associations for Dental Research Online Journals


ARTICLES

Neuraminidase activity: a biochemical marker to distinguish Streptococcus mitis from Streptococcus sanguis

P. A. Murray, M. J. Levine, L. A. Tabak and M. S. Reddy

Selected reference and freshly isolated strains of Streptococcus mitis (mitior) and Streptococcus sanguis were assayed for cell-associated neuraminidase activity by their ability to hydrolyze [3H-] sialyllactitol. A cell-associated neuraminidase was detected with S. mitis and S. sanguis serotype II (reclassified as S. mitis) but not with S. sanguis serotypes I and III. Neuraminidase activity of S. mitis correlated with this organism's inability to hydrolyze arginine, aesculin, and few, if any, sugars. The findings indicate that the presence of cell-associated neuraminidase activity is useful for the taxonomic classification of S. mitis.





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