Journal of Dental Research, Vol 62, 975-979, Copyright © 1983 by International & American Associations for Dental Research Online Journals
The effect of non-steroidal anti-inflammatory drugs on the metabolism of 14C-arachidonic acid by human gingival tissue in vitro
T. M. Elattar, H. S. Lin and D. E. Tira
We investigated the effect of non-steroidal anti-inflammatory drugs on
prostaglandins (PGs) and 12-hydroxyeicosatetraenoic acid (12-HETE)
formation by inflamed human gingival tissues. Gingival tissue homogenates
were incubated with 14C-arachidonic acid in the presence of indomethacin,
piroxicam, or ibuprofen, and the organic solvent extracts were
chromatographed on silica gel plates with standards for radiometric assay.
There was a significant negative trend between the doses (10(-7)-10(-3) M)
of each of indomethacin, piroxicam, and ibuprofen, and the amounts of PGF2
alpha, PGE2, PGD2, and 15-keto-PGE2 produced. All three drugs have a
significant inhibitory effect on PGs and 12-HETE production at 10(-3) M
when compared with the control. The rank order effectiveness of the drugs,
at 10(-3) M, on PG inhibition was indomethacin greater than piroxicam
greater than ibuprofen, and on 12-HETE inhibition was indomethacin greater
than ibuprofen greater than piroxicam.
