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Journal of Dental Research, Vol 62, 917-921, Copyright © 1983 by International & American Associations for Dental Research Online Journals

ARTICLES

Identification of a cathepsin b-like protease in the crevicular fluid of gingivitis patients

D. A. Eisenhauer, R. Hutchinson, T. Javed and J. K. McDonald

Crevicular fluid from gingivitis patients contains significant levels of a cysteine protease which was characterized as the lysosomal protease cathepsin B, as judged by substrate specificity, thiol dependence, pH optimum, kinetic parameters, pH stability, and inhibitor sensitivities. A highly-sensitive fluorometric assay procedure was used to establish the mean level of cathepsin B activity for 25 gingivitis patients.


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