Journal of Dental Research, Vol 60, 164-170, Copyright © 1981 by International & American Associations for Dental Research Online Journals
Ultrastructural alteration of trypsin- and pancreatin-separated embryonic rabbit palate epithelium and mesenchyme
R. J. Lieb and D. P. DePaola
The effects of 30- and 45-minute trypsin- and pancreatin-separation on
embryonic rabbit palate epithelium and mesenchyme were studied with the
electron microscope. Changes in epithelium included fragmentation of the
basal lamina and formation of cytoplasmic blebs associated with the basal
cell layer. Changes in mesenchymal cells were first evident at 45 min of
incubation and included cell fragmentation, nuclear pyknosis, and dilation
of extracellular space. Results indicate a differential susceptibility of
mesenchyme to the separating agent.
