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Journal of Dental Research, Vol 59, 2006-2013, Copyright © 1980 by International & American Associations for Dental Research Online Journals
ARTICLES |
B. Kadis, J. M. Goodson, S. Offenbacher, J. W. Bruns and S. Seibert
An improved method is described for the isolation of osteoblast-like cells from fetal rat calvaria. The cells are mononuclear, 8.3 mu in diameter, alkaline phosphate positive, and possess rough endoplasmic reticulum, mitochondria, Golgi, and cytoplasmic processes. Isolated bone cells synthesize collagen and cholesterol, grow to confluence in primary culture, and respond to hormonal stimulation.
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