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Journal of Dental Research, Vol 58, 1926-1932, Copyright © 1979 by International & American Associations for Dental Research Online Journals

ARTICLES

Calcium-specific precipitation of dentin phosphoprotein: a new method of purification and the significance for the mechanism of calcification

Y. Kuboki, R. Fujisawa, K. Aoyama and S. Sasaki

Calcium, but not magnesium or strontium ions, specifically induce the precipitation of dentin phosphoprotein, which is reversibly solubilized by EDTA. This finding is very useful in pruifying dentin phosphoprotein of either a free or matrix-bound type. Bovine dentin phosphoproteins, thus isolated, contain a minimal amount of contaminant protein, unlike previous preparations.


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