Journal of Dental Research, Vol 58, 1644-1651, Copyright © 1979 by International & American Associations for Dental Research Online Journals
Polyamine metabolism in isoproterenol-stimulated mouse salivary glands
N. F. Nelson, K. B. Brown, D. D. Douthit, S. Ghatan and D. G. Brown
Isoproterenol (0.3 micronmole/gm body weight) was injected
intraperitoneally every 24 h for three days. The synthesis of
deoxyribonucleic acid, the concentration of putrescine, spermidine and
spermine and the activities of ornithine decarboxylase and
S-adenosylmethionine decarboxylase were measured in parotid and
submandibular glands at 4 to 8 h after each injection. The parotid glands
responded with peaks of DNA synthesis at 24 and 72 h and peaks of
putrescine content and decarboxylase activities 8 to 12 h after each
injection. Spermidine increased steadily in the parotid, whereas there was
little change in the spermine concentration throughout the 72 h. Polyamine
metabolism showed much less response in the submandibular gland, and little
or no increase in spermidine or spermine levels or in DNA synthesis was
observed.
