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Journal of Dental Research, Vol 58, 1946-1952, Copyright © 1979 by International & American Associations for Dental Research Online Journals

ARTICLES

Human gingival lymphocytes. I. Methodology for the isolation of human gingival lymphocytes

B. F. Mackler, J. A. Withers, D. L. Woodson, E. Coker, A. Herrin, L. Friedman and P. A. O'Neill

Various methodologies were examined for the isolation of inflammatory cells from diseased human gingiva. The best recovery of viable gingival lymphocytes (gMNC) was achieved by a method which combined initial collagenase digestion followed by gentle teasing with an 18-gauge needle.




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