Journal of Dental Research, Vol 57, 643-649, Copyright © 1978 by International & American Associations for Dental Research Online Journals
Effect of isoprenaline on salivary gland lipids of rats fed a choline-deficient diet
S. Q. Alam and B. S. Alam
Male adult rats were fed choline deficient and a choline-supplemented diet
for 3 weeks. Half the rats from each group were injected with isoprenaline
(16 mg/kg body weight) and the remainder with saline. After 2 and 8 hours,
rats were sacrificed, their submandibular salivary glands were disected
out, and extracted for lipids. The fatty acid composition of total lipids
and some neutral and phospholipid fractions were determined. Isoprenaline
administration resulted in a slightly higher level of oleic acid in SMSG of
rats fed choline-deficient but not the control diet. There was an increase
in the free fatty acid (FFA) concentrations of the gland after 2 hours of
isoprenaline treatment in rats fed the control diet; no such increase was
observed in the choline-deficient group. The fatty acid composition of FFA
fraction was also slightly changed as a result of isoprenaline treatment,
but only in the choline-deficient group. The fatty acid composition of
triglyceride (TG), phosphatidyl choline (PC), and phosphatidyl ethanolamine
(PE) fractions was not changed.
