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Journal of Dental Research, Vol 56, 967-970, Copyright © 1977 by International & American Associations for Dental Research Online Journals

ARTICLES

Proteolipid and bone matrix calcification in vitro

J. Ennever, B. Boyan-Salyers and L. J. Riggan

Proteolipid was demonstrated to contain the nucleator of bone matrix calcification, in vitro. Crude phospholipid extracted from bone matrix was fractionated by gel filtration. A single, protein-containing fraction induced apatite crystallization in a metastable calcium phosphate solution. The fraction was identified as proteolipid. The result supports the validity of a microbiologic analogue for vertebrate calcification.




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