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Journal of Dental Research, Vol 56, 239-248, Copyright © 1977 by International & American Associations for Dental Research Online Journals

ARTICLES

Biochemical and morphological studies of rat submandibular gland: III. effects of testosterone treatment on proteins of granule-rickch fraction

C. T. Hanks and S. G. Chakrabarti

Mature adult male rats and castrated and testosterone-treated castrated adult rats were injected with pilocarpine HCl and 3-H-lysine and sacrificed sequentially over an eight-hour time period. Following homogenization and differential centrifugation, three subcellular fractions from each group of animals were analyzed by gel electrophoresis and liquid scintillation. Two proteins in the granule-rich fraction appeared in larger amounts on the densitometric scans and appeared to represent a larger proportion of newly synthesized proteins than the other proteins.




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