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J Dent Res 54(6): 1138-1142, 1975
© 1975 International and American Associations for Dental Research
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Continuous In Vitro Measurement of Mouse Salivary Gland Enzymes

G. S. SCHUSTER 1, D. H. PASHLEY 1, M. P. PALMER 1, and M. M. SHARAWY 1

1 Departments of Oral Biology, Cell and Molecular Biology, and Physiology, Schools of Dentistry and Medicine, Medical College of Georgia, Augusta, Georgia 30902, USA

A perifusion chamber technique is described that permits continuous measurement of the in vitro release of salivary enzymes from slices of mouse salivary glands responding to various stimuli. Kinetics of amylase secretion differed from those of esterase, which suggests that the secretory processes differ for these two enzymes.

Submitted on April 7, 1975
Accepted on June 27, 1975






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