Glucose Transport in Streptococcus mutans: Preparation of Cytoplasmic Membranes and Characteristics of Phosphotransferase Activity

¹ Microbiology Research Laboratories, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455, USA

Cytoplasmic membranes from Streptococcus mutans were used to study phosphoenolpyruvate (PEP)-dependent glucose transport. The Km values for glucose and PEP were 0.3 and 0.4 mM. The system was sensitive to sulfhydryl reagents and detergents. The nonmetabolizable analogue 2-D-deoxyglucose was readily phosphorylated while -methylglucose and 3-O-methylglucose were inefficiently derivatized. The fermentable sugars fructose, galactose, mannitol, and sorbitol were phosphorylated at reduced rates.

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