Bone Lipids: In Vitro Synthesis in Bicarbonate and Phosphate Buffers

¹ Departments of Oral Biology and Biochemistry, Schools of Dentistry and Medicine, Medical College of Georgia, Augusta, Georgia 30902, USA

Calvaria from new-born rats were incubated with ¹⁴C-acetate in Krebs-Ringer bicarbonate or phosphate buffer of varying pH to study lipid synthesis. Lipid radioactivity was always greater in bones incubated in phosphate buffer. No optimum pH value was observed between pH 6.5 and 7.5.