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1 Department of Microbiology, West Virginia University Medical Center, Morgantown, West Virginia 26506, USA
Calculus specimens were examined by direct fluorescent antibody techniques and cultural procedures for the presence of gram-positive filamentous or diphtheroid bacteria. Actinomyces israelii, A viscosus, A naeslundii, Arachnia propionica, Rothia dentocariosa, Bacterionema matruchotii, and Corynebacterium acnes were observed in and cultured from the majority of specimens, with more than one species present in every specimen.
Submitted on February 3, 1970
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