Observations on Oral Tumors in Vitro. 2 Karyobiosis in Strain KB Cells

¹ Department of Medicine (Periodontics), University of Texas Dental Branch: Department of Biology University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas; and Department of Cellular Biology, Pasadena Foundation for Medical Research, Pasadena, California

Nuclei of strain KB cells growing on glass coverslips in tissue culture chambers were shown by time-lapse techniques to exist in a variety of forms and to undergo marked morphological and positional transformations. Giant nuclei were observed regularly. Generally, they contained prominent nuclear folds and intranucleolar droplets and, in some instances, inclusions of cytoplasm.

Three forms of nuclear rotation were illustrated. Time-lapse sequences showed the plasticity and secretary processes of the nucleoli. The multinucleation by mitosis in a giant cell revealed a new geometrical form of metaphase chromosomes.

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