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J Dent Res 43(2): 246-251, 1964
© 1964 International and American Associations for Dental Research
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Quantitative Determination of Some Constituent Lipids in Human Dentin

THOMAS R. DIRKSEN 1 and KENNETH G. IKELS 1

1 School of Aerospace Medicine, USAF, Brooks Air Force Base, Texas

A method is described for the extraction of dentin lipids and the separation of those lipids into six fractions: cholesterol esters, free cholesterol, triglycerides, diglycerides, monoglycerides, and phospholipids.

Essentially, the method requires the decalcification of dentin using EDTA and subsequent extraction with diethyl ether, ethanol, chloroform, and methanol. The lipid extract is separated into six components by a small silicic acid column and the effluents are quantitated colorimetrically.

Submitted on April 4, 1963




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 Crit. Rev. Oral Biol. Med.Home page
M. Goldberg and D. Septier
PHOSPHOLIPIDS IN AMELOGENESIS AND DENTINOGENESIS
Crit. Rev. Oral. Biol. Med., May 1, 2002; 13(3): 276 - 290.
[Abstract] [Full Text]


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