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J Dent Res 42(3): 852-857, 1963
© 1963 International and American Associations for Dental Research
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Hydroxylapatite Chromatography of Cholinesterases That Hydrolyze Local Anesthetics

CHARLES E. BECKER 1

1 American Dental Association, Chicago, Illinois

Human serum was chromatographed on hydroxylapatite. The eluates were assayed for activity against acetylcholine, metabutethamine, meprylcaine, piperocaine, procaine, butethamine, and tetracaine. The results show the presence of several cholinesterases in human serum. These enzymes vary in their ability to hydrolyze the estertype dental local anesthetics. The relative distribution of cholinesterase activity in serum differs from that of a concentrated plasma cholinesterase.*

Submitted on October 2, 1962






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