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J Dent Res 41(6): 1383-1385, 1962
© 1962 International and American Associations for Dental Research
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Microbiologic Mineralization: A Calcifiable Cell-free Extract from a Calcifiable Micro-organism

J. ENNEVER 1

1 Ivorydale Technical Center, Procter & Gamble Company, Cincinnati, Ohio

The purpose of this study was to determine whether a calcifiable, cell-free extract could be prepared from a micro-organism known to form intracellular calcium hydroxyapatite. The objective was accomplished by subjecting the cells to an acetic acid extraction with recovery of the extract by water dialysis. The cell-free extract was suspended in calcifying solution for 10 days. X-ray diffraction revealed that the extract had acquired calcium hydroxyapatite. Freeze-dried extract failed to induce hydroxyapatite formation.

Submitted on April 14, 1962






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