Tissue Culture Characteristics of the BW1081 Mouse Salivary Gland Tumor. I. Preliminary Investigation of Nutrient Medium Components

¹ Departments of Oral Pathology and Pathology, Indiana University Schools of Dentistry and Medicine, Indianapolis, Indiana

Growth characteristics of the BW1081 mouse salivary gland tumor cells were tested in a tissue culture system by systematically eliminating each component of the nutrient medium and determining the effect on cell proliferation by means of actual viable cell counts. Elimination of individual amino acids, individual components of Earle's basal salt solution, or of lactalbumin hydrolyzate or egg ultrafiltrate resulted in general growth failure. The addition of individual hormones, RNA and DNA at certain levels resulted in cytotoxic effects, while at other levels certain degrees of stimulation of cell proliferation were noted. This stimulation was most pronounced with purified growth hormone.