Standardized Paper Electrophoresis of Parotid Saliva A Study of Variables in Technique and Flow Rate as Factors in Resolution

¹ Murry and Leonie Guggenheim Foundation, Institute for Dental Research, New York University College of Dentistry, New York

A routine procedure for paper electrophoresis has been developed for parotid saliva in veronal buffer, pH 8.6, µ, 0.05. Samples were dialyzed and concentrated by pervaporation, prior to analysis. Electrophoresis was carried out for 17 hours at 300 volts, 4-4.5 ma. as constant voltage, and the strips were stained with Nigrosine.

This standardized technique was checked for reproducibility to show the constancy of the number of fractions produced by electrophoretic resolution, the relative positions of these fractions in repeated runs of the sample, the total pattern picture characteristic of and obtained with each particular sample. While some slight variations due to staining were recognized, an entirely satisfactory reproducible technique has been evolved.

A study of 50 subjects has shown that a flow rate greater than 1 ml/min for a single parotid gland appears to be required for adequate electrophoretic studies. This factor appears to be related to gland function rather than to the conditions for electrophoretic analysis, already established as routine. Further investigations of these electrophoretic components are now in progress in our laboratory.

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