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1 Special Dental Research Laboratory, Veterans Administration Hospital, Brooklyn, New York, and the Murry and Leonie Guggenheim Foundation, Institute of Dental Research, New York University College of Dentistry, New York, N.Y.
A correlated cytochemical and biochemical study has been made of aerobic oxidase activity of human gingival slices and homogenates. Indophenol blue synthesis by fresh-frozen sections was used as a means of assessing relative oxidation capacities of different cell types of the gingival epithelium. Crevicular basal cells were the first to synthesize the dye, followed by basal cells of the attached gingiva. Color development then proceeded outward to surface layers of the tissue. Parakeratin and keratin stained deeply after 60-90 minutes' incubation in the reagent mixture. Indophenol blue could not be leached out of the keratin or parakeratin by a large number of solvents used for this purpose with other tissues. In manometric assays of cytochrome oxidase activity of gingival homogenates, QO2 (N) values between 17 and 25 were obtained, corresponding to QO2 (dry-weight) values of 2.7-4.0. Some preparations were completely inactive manometrically. In beginning experiments, addition of gingival homogenates to active heart-muscle cytochrome oxidase preparations inhibited activity of the oxidase.
Submitted on June 6, 1960
This article has been cited by other articles:
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  J. Felton, P. Person, and S. S. Stahl Biochemical and Histochemical Studies of Aerobic Oxidative Metabolism of Oral Tissues. IV. Additional Observations on Histochemical Oxidase Reactions in Gingiva Journal of Dental Research, November 1, 1965; 44(6): 1238 - 1243. [PDF]
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P. Person, A. Fine, and J. Felton Biochemical and Histochemical Studies of Aerobic Oxidative Metabolism of Oral Tissues. V. Visible and Soret Absorption Spectra of Gingival and Tongue Preparations Journal of Dental Research, November 1, 1965; 44(6): 1244 - 1246. [PDF]
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J. H. Felton, P. Person, and S. S. Stahl Biochemical and Histochemical Studies of Aerobic Oxidative Metabolism of Oral Tissues. II. Enzymatic Dissection of Gingival and Tongue Epithelia from Connective Tissues Journal of Dental Research, March 1, 1965; 44(2): 392 - 401. [PDF]
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P. Person, J. Felton, and A. Fine Biochemical and Histochemical Studies of Aerobic Oxidative Metabolism of Oral Tissues. III. Specific Metabolic Activities of Enzymatically Separated Gingival Epithelium and Connective-Tissue Components Journal of Dental Research, January 1, 1965; 44(1): 91 - 95. [PDF]
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B. Eichel, M. A. Shahrik, and V. F. Lisanti Cytochemical Demonstration and Metabolic Significance of Reduced Diphospho-Pyridinenucleotide and Triphospho-Pyridinenucleotide Reductases in Human Gingiva Journal of Dental Research, January 1, 1964; 43(1): 92 - 106. [PDF]
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P. Person Relation of Metabolism of Oral Tissues to Periodontal Disease Journal of Dental Research, January 1, 1963; 42(1): 497 - 501. [PDF]
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