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J Dent Res 40(2): 249-257, 1961
© 1961 International and American Associations for Dental Research
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Effects of Heat and Sonic Oscillation on Cytochrome c

PHILIP PERSON 1 and ALBERT FINE 1

1 Special Dental Research Laboratory, Veterans Administration Hospital, Brooklyn, New York

A study was made of the effects of exposure of solutions of cytochrome c to 100° C. for 2 hours and to sonic oscillation (9 kc) for 5 hours. The primary changes revealed by spectrophotometric and manometric determinations in both types of experiment were an irreversible loss of solubility, denaturation of the enzyme, and loss of catalytic activity. In addition, sonic oscillation produced a very fine black sediment, not seen as a result of heat treatment.

Submitted on April 27, 1960






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