Methods of Preparing Stained Ground Sections of Soft and Hard Tissues

¹ Department of Anatomy, Schools of Dentistry and Medicine, Hebrew University-Hadassah, Jerusalem, Israel

Methods for processing simultaneously both soft and hard tissues for histologic study are described. A commercial polyester resin and methyl methacrylate were used for imbedding, either individually or in a double-imbedding technique. Manual or mechanical grinding was employed for obtaining single or serial sections. The various staining techniques applicable to these sections are given, and the results obtained are illustrated.