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J Dent Res 39(5): 1041-1048, 1960
© 1960 International and American Associations for Dental Research
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In Vitro Calculus Formation

FRITHJOF VON DER FEHR 1 and FINN BRUDEVOLD 1

1 Forsyth Dental Infirmary and Harvard School of Dental Medicine, Boston, Massachusetts

An in vitro technique is reported which permits independent study of plaque formation and calcification on a quantitative basis.

Maintenance of salivary CO2 at 60 mm. Hg and pH below 7.2 during plaque formation inhibited in vitro formation of calculus. Because decalcified calculus was shown to remineralize, it was concluded that the organic matrix contained a substance which could initiate calcification.

Submitted on February 12, 1960
Revised on April 8, 1960




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B. N. Bachra, O. R. Trautz, D. McConnell, W. J. Frajola, and D. W. Deamer
Carbonic Anhydrase and the Precipitation of Apatite
Science, August 3, 1962; 137(3527): 337 - 338.
[Abstract] [PDF]


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