An Improved Technique for the Paper Electrophoresis of Parotid Saliva

¹ Department of Microbiology, School of Dentistry, University of Pennsylvania

Preliminary work indicated that application of electrophoresis designed for serum analysis is unsatisfactory when applied to the analysis of human parotid saliva. The main variation from other published techniques was the use of a 30-minute period which allowed the paper strips to attain an optimum amount of dampness. The most favorable results were obtained by conducting the electrophoresis at 21° C. and staining the strips with amidoschwartz l0b. By the use of this method it is possible to obtain clear-cut and distinct separations of eleven components in parotid saliva.