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J Dent Res 38(2): 323-327, 1959
© 1959 International and American Associations for Dental Research
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AMINO ACID COMPOSITION OF PROTEINS OF HUMAN DENTIN

HUBERT H. HUGHSTON 1, LEWIS S. EARLE 1, and FRANCIS BINKLEY 1

1 Department of Biochemistry, Emory University, Atlanta, Ga.

The insoluble protein of dentin was found to be a collagen characterized by a high content of glycine, proline, and hydroxyproline; the presence of hydroxylysine and hydroxyproline confirms the classification as a collagen. The discovery of glutamic and aspartic acids in peptides in the acid hydrolysates is novel and may be a clue as to the combination of the collagens in the organic matrix of dentin. The collagen fraction of dentin was found to contain more of the hydroxyaliphatic amino acids, hydroxyproline, and hydroxylysine, and less of the basic amino acids, arginine and lysine, than collagens from other sources.

Submitted on August 11, 1958
Revised on January 19, 1959






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