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J Dent Res 37(4): 738-747, 1958
© 1958 International and American Associations for Dental Research
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EXPERIMENTAL STUDIES ON CALCIFICATION OF TOOTH GERMS IN VITRO

SHIRLEY GLASSTONE, L.D.S. PH.D. (Cantab)1

1 Anatomy School, University of Cambridge, Cambridge, England

1. Whole tooth germs, caps, and formed matrix of rats, mice, and hamsters have been calcified in vitro.

2. In the caps and the formed matrix, cells in which alkaline phosphatase is present have been removed and yet the dentin and enamel matrix calcified, so intracellular alkaline phosphatase does not appear to play a part in calcification.

3. Beryllium and protamine added to the calcifying solutions inhibit calcification by competing with the calcium salts and preventing its deposition in the matrix.

4. Caps digested with testicular hyaluronidase calcified in the solutions.

5. Chondroitin sulfate does not play an important part in the calcification of dentin.

Submitted on January 1, 1958
Revised on February 3, 1958






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