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1 Department of Dentistry and Dental Research, Eastman Dental Dispensary, University of Rochester, Rochester, N. Y.
1. A histologic study of brown pigmentation in enamel surfaces in relation to tooth age has been made on ground sections from 82 teeth.
2. Microscopic study of predetermined areas of gingival, middle, and occlusal thirds of enamel surfaces showed a consistent increase in the amount and intensity of surface pigmentation with increasing age.
3. Decalcification under the microscope of some sections demonstrated that the pigmented areas are highly resistant to the action of acids.
4. It is suggested that the material making up the pigmented areas is basically organic in nature having its origin in the saliva.
Submitted on December 1, 1955
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