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1 Departments of Biochemistry and Bacteriology, Dental School, University of Oregon, Portland, Ore.
A rapid, quantitative method for estimation of salivary alpha-amylase has been developed. A known amount of starch is incubated with 0.1 ml. of saliva and the amount remaining is determined photocolorimetrically by development of the starch-iodine blue complex.
The amount of human salivary alpha-amylase present is determined by comparison with the action of commercial amylase under the same conditions.
Human salivas can be quickly grouped into 3 arbitrary groups in respect to their amylase content.
It is possible to prepare all the reagents, with the exception of the diluted working solution of iodine, at least 24 hours in advance of the time they are to be used.
Two technicians can analyze 150 samples of saliva per day.
There is no correlation between amounts of salivary alpha-amylase and lactobacillus counts, acid production in selective media or volume of respective specimens of saliva.
Submitted on December 8, 1954
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