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J Dent Res 35(2): 260-262, 1956
© 1956 International and American Associations for Dental Research
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STUDIES OF THE COMPOSITION OF TEETH

IV. THE AMINO ACID COMPOSITION OF HUMAN ENAMEL PROTEIN

GINO C. BATTISTONE PH.D.1 and GEORGE W. BURNETT D.D.S., PH.D.1

1 Department of Dental Research, Army Medical Service Graduate School, Walter Reed Army Medical Center, Washington, D. C.

The concentration of 19 amino acids in the protein residue of ethylenediamine tetraacetic acid (EDTA) decalcified human enamel was determined by quantitative two-dimensional paper chromatography. The amino acids accounted for 99.21 per cent of the total nitrogen of enamel. The histidine: lysine:arginine ratio of enamel is 1:4:12, which is typical of eukeratins. Since enamel protein contains proline and hydroxyproline, and much glycine and little cystine, its composition is not typical of true keratins.






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