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J Dent Res 35(2): 255-259, 1956
© 1956 International and American Associations for Dental Research

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STUDIES OF THE COMPOSITION OF TEETH

III. THE AMINO ACID COMPOSITION OF HUMAN DENTINAL PROTEIN

GINO C. BATTISTONE PH.D.1 and GEORGE W. BURNETT D.D.S., PH.D.1

1 Department of Dental Research, Army Medical Service Graduate School, Walter Reed Army Medical Center, Washington, D. C.

The amino acid composition of human dentinal protein residues, obtained by decalcification with ethylenediamine tetraacetic acid (EDTA) and by refluxing in distilled water, was determined by quantitative, two-dimensional paper chromatography. Twenty amino acids were found, and the composition of the 2 residues was quite similar. The amino acids of EDTA decalcified dentin and water extracted dentin accounted for 98.18 per cent and 97.01 per cent of the respective total nitrogen. The amino acid composition of dentin is quite similar to that of collagen.







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