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J Dent Res 28(5): 525-526, 1949
© 1949 International and American Associations for Dental Research
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DECALCIFICATION OF BONE AND TEETH UNDER VACUUM—A RAPID METHOD FOR PRODUCING HARD TISSUE PREPARATIONS

JENS WAERHAUG L.D.S.1

1 Histo-Pathological Department, State Dental Institute of Stockholm, Stockholm, Sweden

By performing the decalcification under vacuum it has been possible to reduce the time required for this process to one-tenth. The rate of the process is proportional to the vauum. For that reason it is recommended to use a rather high degree of vacuum (about 74 cm. Hg.)

The quality of the slides obtained in this way is usually better, no disadvantages have been observed after using the method for 6 months.

It is pointed out that nitric acid usually is applied in a concentration more than three times stronger that trichloracetic acid. This may explain the faster decalcification with nitric acid and the better result obtained with trichloracetic acid.

Submitted on December 20, 1948






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