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J Dent Res 27(1): 41-45, 1948
© 1948 International and American Associations for Dental Research

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A METHOD FOR QUANTITATIVE DETERMINATIONS OF MICROORGANISMS IN CARIOUS AND NON-CARIOUS TEETH OF THE COTTON RAT

ELIZABETH J. WAKEMAN 1, J. KNOX SMITH 1, WILLIAM B. SARLES 1, PAUL H. PHILLIPS 1, and C. A. ELVEHJEM 1

1 Departments of Biochemistry and Agricultural Bacteriology, College of Agriculture, University of Wisconsin, Madison, Wis.

A method is described for determining the total bacterial count of carious teeth in the cotton rat. Bacterial counts made by this method show conclusively that the greater numbers of organisms in various teeth are located in the deep-fissure lesions and may only be determined by grinding and culturing the entire tooth.

Submitted on October 11, 1947







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