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J Dent Res 26(4): 327-332, 1947
© 1947 International and American Associations for Dental Research
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THE SELECTION OF A SUITABLE MEDIUM FOR CULTURING ROOT CANALS

DONALD E. SHAY PH.D.1

1 University of Maryland, School of Dentistry, Baltimore, Md.

In 184 positive root canals cultured, 97.8% were positive on trypticase dextrose broth, pH 7.2, and brain heart infusion, pH 7.4. This result proved these 2 media to be sufficient for practical purposes.

The frequency of the appearance of these organisms was found to be in this order: Streptococcus gamma type, Staphylococcus albus, Streptococcus beta type, Bacillus subtilis, Streptococcus alpha type, Staphylococcus aureus, Lactobacillus acidophilus, yeast, and other miscellaneous organisms.

Cultures of positive root canals are not all pure; there are a few mixed cultures.

Strict anaerobic conditions are not necessary for cultivation of bacteria from root canals

Submitted on May 24, 1947






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