SALIVA INCUBATED WITH GLUCOSE AS A REPLACEMENT FOR TRYPTOPHANE IN A SYNTHETIC MEDIUM SUPPORTING THE PRODUCTION OF ACID BY LACTOBACILLUS ACIDOPHILUS

¹ Harvard School of Dental Medicine, Boston, Mass.

1. Human saliva incubated with glucose at 37.5° C. for 24 or more hours may be substituted for the added tryptophane in a synthetic medium which promotes the production of acid by Lactobacillus acidophilus.

2. Saliva incubated without added glucose caused only a minimal increase in the production of acid.

3. When saliva incubated with glucose was substituted for casein hydrolyzate in the synthetic medium acid production occurred but the quantity was only about one-fifth that obtained in the presence of the casein hydrolyzate.