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1 Harvard School of Dental Medicine, Boston, Mass.
Specimens of Lactobacillus acidophilus isolated from the mouths of 23 clinic patients having active dental caries have been shown to have uniform morphological and biochemical characteristics. This organism has been grown successfully on the medium of Landy and Dickens which consists almost entirely of purified chemical substances. The quantity of acid produced in standardized experiments was closely reproducible. Enrichment of the media by addition of extra quantities of each individual constituent was not accompanied by any significant increase in the production of acid. Omission of certain of these constituents caused almost complete cessation of acid production while others have been found to be non-essential.
A medium consisting only of glucose, tryptophane, sodium acetate, potassium phosphates, thiamin hydrochloride, calcium pantothenate, nicotinic acid and vitamin free casein hydrolysate is capable of providing for maximum acid production with this oral strain of Lactobacillus acidophilus.
Submitted on December 29, 1945
This article has been cited by other articles:
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  D. WEISBERGER Role of Glucose in Promoting Growth of Lactobacilli in Saliva Science, March 14, 1947; 105(2724): 290 - 290. [PDF]
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