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J Dent Res 24(3): 143-153, 1945
© 1945 International and American Associations for Dental Research
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FORMIC ACID-SODIUM CITRATE DECALCIFICATION AND BUTYL ALCOHOL DEHYDRATION OF TEETH AND BONES FOR SECTIONING IN PARAFFIN

ANNA MORSE M.S.1

1 Department of Oral Pathology, Tufts College Dental School, Boston, Mass.

A technic for the preparation of oral calcified tissue for histological examination ion is presented. This technic differs from other available methods in combining 3 steps which are (1) decalcification by means of a formic acid-sodium citrate reagent, (2) dehydration with n-butyl alcohol, and (3) embedding in paraffin. Excellent sections at 7 microns were obtained following employment of the method on rat, dog, and human jaws. Other calcified tissue has been successfully treated in the same manner. Celloidin embedding is used only when the pieces of human or dog tissue exceed 3 teeth in situ.

Submitted on March 2, 1945






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