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J Dent Res 22(1): 45-51, 1943
© 1943 International and American Associations for Dental Research
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A QUANTITATIVE METHOD FOR EVALUATING PHYSICAL AND CHEMICAL AGENTS WHICH MODIFY PRODUCTION OF ACIDS IN BACTERIAL PLAQUES ON HUMAN TEETH

ROBERT M. STEPHAN M.S., DD.S.1 and BENJAMIN F. MILLER CH.E., M.D.1

1 Walter G. Zoller Memorial Dental Clinic and the Department of Medicine, University of Chicago, Chicago, Ill.

A method is described which gives a quantitative expression of the intensity and duration of the hydrogen-ion changes in situ occurring in bacterial plaques on human teeth after the application of a solution of glucose. The proposed method depends on the demonstration that the pH curves obtained after each of 2 glucose rises given 30 minutes or more apart are almost exact duplicates. Therefore the effect of any experimental procedure which inhibits or neutralizes the formation of acid by plaques can be evaluated by introducing the experimental procedure after completion of the first pH curve and before the start of the second one. The difference in the areas of the pH curves gives a measure of the inhibitory effect of the experimental procedure.

Submitted on September 14, 1942




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