VITAL STAINING OF GROWING BONES AND TEETH WITH ALIZARINE RED S
I. SCHOUR D.D.S., PH.D.1,
M. M. HOFFMAN D.D.S., M.S.1,
B. G. SARNAT M.D., D.D.S.1, and
M. B. ENGEL D.D.S., M.S.1
1 Department of Histology, University of Illinois, College of Dentistry, Chicago, Ill.
An intraperitoneal injection of a saturated solution (2 per cent) of alizarine Red S at a dosage of 50-100 mg. per kg. produces a sharp red line in the dentin and bone calcifying at the time. This method permits the measurement of the rate of growth of calcifying structures in normal and pathologic conditions in various experimental animals. Effective doses can be given without significant toxic complications.
Submitted on April 17, 1941
