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1 Department of Operative Dentistry, University of Illinois, Chicago
Smears taken from the surface of the mucous membrane of the mouth were used to study the cytologic detail of the superficial epithelial cells. The staining method of Ernst-Gram revealed a series of six stages in the process of keratinization. The degree of keratinization was lowest in the smears from the cheek; it increased progressively in the smears from the tongue, gingivae and palate. The number of leucocytes in the smears decreased gradually with an increase in the degree of keratinization of the epithelial cells.
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  H. Hoffman and J. C. Simon Gram-Staining Mechanism of Superficial Oral Epithelial Cells Journal of Dental Research, May 1, 1969; 48(3): 392 - 395. [PDF]
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E. R. Zimmermann and A. L. Zimmermann Effects of Race, Age, Smoking Habits, Oral and Systemic Disease on Oral Exfoliative Cytology Journal of Dental Research, July 1, 1965; 44(4): 627 - 631. [PDF]
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C. G. Bennett Study of Exfoliative Cytology of Oral Mucosa of Children Exhibiting Clinical Evidence of Ectodermal Dysplasia Journal of Dental Research, July 1, 1963; 42(4): 943 - 949. [PDF]
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