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1 Department of Biochemistry and Pharmacology, School of Medicine and Dentistry, The University of Rochester, Rochester, New York
1. A centrifugal method has been devised for the routine separation and purification of enamel and dentin in single teeth, making use of a heavy liquid (density - 2.7 gm. per cc.) and a new type of separation tube.
2. A single separation requires about 1 hour, entails a total loss of material of about 3 per cent and gives enamel and dentin of 99 per cent purity as determined by a refractive index method.
3. Enamel of 99.4 per cent purity may be obtained by a second separation using pure bromoform. About 9 per cent further loss of enamel occurs.
4. Dentin of 99.7 per cent purity may be obtained by a second fractionation using a liquid of density 2.42. About 6 per cent further loss of dentin occurs.
5. Cementum may be removed from the dentin by the use of a liquid of density 2.07.
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