Figure 1. BSE analysis (a–c) and light micrographs of sections stained by H-E (d–f) or immunostained for GFP (g,h) or BrdU (i–l). Transplanted pulp after 3 (d,i), 7 (a,e,j), and 14 (b,f,g,h,k) days. (a) Calcified hard tissue of the inner boundary of the pulp is evident. (b) Most of the pulp tissue is calcified. (c) The area of mineralized tissue was measured from images obtained by BSE analysis. (d) Typical odontoblasts have disappeared (asterisks) in the periphery of the pulp. (e) Extracellular matrix (stars in e–g,j,k) has formed within the peripheral pulp. (f) Matrix formation extends into the pulp. (g,h) GFP-positive cells are detected within the matrix and on its surface. "h" is an enlargement of the area denoted by the rectangle in "g", with the arrowheads pointing to GFP-positive cells on the surface of the matrix. (i) BrdU-positive cells are not seen in the pulp. (j) Numerous positive cells are apparent in the center of the pulp. (k) Positive cells have decreased in number. (l) Number of BrdU-positive cells counted in the immunostained sections. (c,l) Data are expressed as the means ± SE from 5 specimens per group. ANOVA and Student’s t test show a significant difference between the 2 groups. p values < 0.05 are considered statistically significant. The boundary between the transplant and the surrounding connective tissue (CT) is indicated by the dotted line (d–g, i–k). BV, blood vessel. Scale bar: 300 µm (a,b), 120 µm (g, i–k), 100 µm (d–f), 40 µm (h).