Figure 4. 3D representation of changes in fluorescence of FURA 2 AM in (a) the control group (n = 9), (b) the nifedipine group (n = 8), and (c) the cyclosporin group (n = 6). Notice the inhomogeneous distribution of cytoplasmic fluorescence (and therefore of calcium ions) in (a), (b), and (c). Increment in fluorescence is expressed as F/F. Changes in ratiometric values of fluorescence, thought to be proportional to intracellular free calcium, are shown in (d). The nifedipine group showed the lowest [Ca²⁺]_i, but not significantly different from that in the control group. The cyclosporin group showed the highest cytoplasmic calcium concentration under resting conditions (*p < 0.05). The data are expressed as the mean ± SD.