Figure 2. Western blot of extracts from Amelx null/M180, Amelx null/P70T, and Amelx null first molars. Anti-amelogenin antibody was used to detect amelogenin protein. Lanes: KOxM180-31, KOxP70T-2, or KOxP70T-16 indicates female null and male transgenic parents. M– is null male without transgene; M+ is null male with transgene. F– and F+ are heterozygous females without and with the transgene, respectively.