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Figure 4


Figure 4. IL-6, IL-8, and MCP-1 production induced by TLR and NOD ligands in human gingival fibroblasts was mediated by NF-{kappa}B. (a) Fibroblasts were incubated for 24 hrs in the presence or absence of FSL-1 (1 nM), Poly I:C (10 µg/mL), lipid A (10 ng/mL), ssPolyU (10 µg/mL), CpG DNA (1 µM), FK156 (10 µg/mL), or MDP (10 µg/mL). IL-6, IL-8, and MCP-1 levels in the culture supernatants were determined with ELISA, and expressed as means ± SD. *Values marked differed significantly from those with medium alone (none). The results presented are representative of 3 different experiments demonstrating similar results. (b) Gingival fibroblasts transfected with siRNA targeting NF-{kappa}B p65 for 24 hrs were stimulated with FSL-1 (1 nM), Poly I:C (10 µg/mL), lipid A (10 ng/mL), ssPolyU (10 µg/mL), CpG DNA (1 µM), FK156 (10 µg/mL), or MDP (10 µg/mL). After 24 hrs of stimulation, the IL-8 levels in the culture supernatants were determined with ELISA, and expressed as means ± SD. *,#Values marked differed significantly from those with medium alone or respective cultures stimulated with the indicated ligands, respectively. The results presented are representative of 4 different experiments demonstrating similar results.